Ultrastructural changes in goat interspecies and intraspecies reconstructed early embryos

Ultrastructural changes in goat interspecies and intraspecies reconstructed early embryos.

The low efficiency of somatic cell nuclear transfer may be related to the ultrastructural deviations of reconstructed embryos. The present study investigated ultrastructural differences between in vivo-produced and cloned goat embryos, including intra- and interspecies embryos. Goat ear fibroblast cells were used as donors, while the enucleated bovine and goat oocytes matured in vitro as recipi...

Ultrastructural changes in preimplantation rabbit embryos.

Follicular oocytes and penetrated eggs of several mammalian species have been the subject of numerous electron microscope studies (rat: Sotelo 1959, Sotelo and Porter 1959, Franchi 1960, Odor and Renninger 1960, Szollosi and Ris 1961, Guinea pig: Anderson and Beams 1960, Adams and Hertwig 1964, Austin 1965. Barron and Franklin 1968. cat: Liss 1964. man: Stegner and Waternberg 1961, Zamboni et a...

Intraspecies and Interspecies Transformation Reactions in Pneumococcus and Streptococcus

The efficiency of transformation of pneumococcus and a strain of viridans streptococcus (strain D) to streptomycin resistance is influenced by the species in which the mutation to resistance occurred, as well as by the species in which the mutated gene has been replicated. Pneumococcus and streptococcus strain D transform in higher frequency with DNA that has been replicated in bacteria of the ...

Ultrastructural changes of embryos in wheat infected with storage fungi.

Effect of Fresh and Frozen Semen on in vitro Fertilization and Subsequent Development of Goat Embryos

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...